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HPTLC METHOD FOR ESTIMATION OF PHYLLANTHIN IN PHYLLANTHUS AMARUS AND ITS MARKET POLYHERBAL FORMULATIONS

Abstract no :  4-1..4-BK-036
Author(s) :   Patel, K.N.; Shah, U.D.; Patel, H.D.; Shah B.K
Address :   (Department of Pharmacognosy, L.M. College of Pharmacy, Ahmedabad, Gujarat, India)
Source :   Proceedings of International Congress on "Ayurveda-2000", Chennai, TN, India, p. 188-189, 28-30 January 2000
Title :  HPTLC METHOD FOR ESTIMATION OF PHYLLANTHIN IN PHYLLANTHUS AMARUS AND ITS MARKET POLYHERBAL FORMULATIONS
Abstract :        A precise, simple and reproducible HPTLC method for the determination of phyllanthin in Phyllanthus amarus and its market polyherbal formulations was developed using tolueneethylacetate (4:2 V/V) as mobile phase and scanned at 280 nm. The sensitivity found for HPTLC was 50 ng and linearity was observed in the range of 200-10000 ng. The leaf contain maximum amount of phyllanthin (0.5449 percent) followed by herb (0.485 percent), stem (0.013 percent) and root (0.0049 percent). The market formulations were evaluated for the percent of phyllanthin on the basis of 0.485 percent of phyllanthin in herb.

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