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SPECTROSCOPIC ANALYSIS OF THE CYTOAGGLUTINATING ACTIVITY OF ABRIN-B ISOLATED FROM ABRUS PRECATORIOUS SEEDS AGAINST LEUKEMIC CELL.

Abstract no :  2-1&2-DG-066
Author(s) :  Ohba, H.; Toyokawa, T.; Yasuda, S.; Hoshino, T.; Itoh, K.; Yamasaki, N.
Address :  Kyushu National Industrial Research Institute, Shuku-Machi, Tosu, Saga 841, Japan
Source :  Bioscience, Biotechnology and Biochemistry,v. 61(4): p. 737-739, 1997.
Title :  SPECTROSCOPIC ANALYSIS OF THE CYTOAGGLUTINATING ACTIVITY OF ABRIN-B ISOLATED FROM ABRUS PRECATORIOUS SEEDS AGAINST LEUKEMIC CELL.
Abstract :      The cytoagglutinating activity of abrin-b, a toxic lectin isolated from Abrus Precatorius seeds, (Gunja) against cultured cell strains derived from  accute lymphoblast leukemia was investigated by visible spectroscopy.  Upon addition of abrin-b, the turbidity at 600nm of cell suspension decreased and this change could be recorded as the cytoagglutination curve.  Fromthis curve, the cytoagglutination velocity (CV) cytoagglutination intensity (CI) of each cell strain was measured.  Each cell strain showed the respective CV and CI values and the cell strains derived from the T cell line were strongly agglutinated by abrin-b compared with those derived from the B cell line.  Further, it has become apparent that the cytoagglutinating activity increased with an increase in the order of the differentiation of cell strains.

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